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super script iii reverse transcriptase kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher super script iii reverse transcriptase kit
    Super Script Iii Reverse Transcriptase Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/super-script+iii+reverse+transcriptase+kit/pmc11346181-331-10-16
    Average 86 stars, based on 1 article reviews
    super script iii reverse transcriptase kit - by Bioz Stars, 2026-11
    86/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Behavior of Mn-sod, PAL1, aos1 and HPL genes in soybean plants overexpressing the NmDef02 defensin
    Article Snippet: The cDNA was synthesized from 1μg of total RNA using an oligo-(dT) primer and the Super-Script III reverse transcriptase kit (Invitrogen, United States) according to the manufacturer’s instructions.

    Article Title: ABI3, a component of the WAVE2 complex, is potentially regulated by PI3K/AKT pathway
    Article Snippet: The full length cDNA of ABI3 (accession number NM_016428.2) was synthetized from a RNA isolated from a FTA sample using using Super-Script III Reverse Transcriptase kit with an oligo(dT)12–18 primer and 10 units of RNase inhibitor (Invitrogen, Life Technologies, Grand Island, NY), according to the manufacturers' recommendations.

    Article Title: α V integrins in Schwann cells promote attachment to axons, but are dispensable in vivo
    Article Snippet: RNA was isolated from purified SCs derived from desheathed sciatic nerves of Itga5 f/f ; Itga5 f/f or Itga5 f/f ; Itga5 f/f ; P 0 - Cre tg/ + postnatal day (P)17 and P6 mice using RNeasy Plus Micro Kit (Qiagen) and TRIzol (Thermo Fisher), respectively. cDNA was synthesized using Super-Script III Reverse Transcriptase kit (Life Technologies).

    Article Title: Aneuploidy as a cause of impaired chromatin silencing and mating-type specification in budding yeast
    Article Snippet: RNA was extracted as described above and cDNA was prepared from 2 μg of the resulting total RNA using the Super-Script III reverse transcriptase kit (Thermo Fisher Scientific, Waltham, MA).

    Article Title: Targeting the PRC2-dependent epigenetic program alleviates urinary tract infections
    Article Snippet: For quantitative real-time PCR (qRT-PCR) experiments, mRNA was reverse-transcribed into cDNA using the Super-Script III Reverse Transcriptase Kit (Invitrogen). qRT-PCR analyses were performed using SYBR Green (Roche) on an ABI-7500 detector (Applied BioSystems).

    Article Title: Alteration of circulating redox balance in coronavirus disease-19-induced acute respiratory distress syndrome
    Article Snippet: A260/A280 > 2 was evaluated to guarantee protein-free samples. cDNA was obtained using a random hexamer primer and a Super-Script III Reverse Transcriptase kit as described by the manufacturer (Invitrogen, Frederick, MD, USA).

    Article Title: α V integrins in Schwann cells promote attachment to axons, but are dispensable in vivo
    Article Snippet: Real-time quantitative PCR RNA was isolated from purified SCs derived from desheathed sciatic nerves of Itga5 f/f ; Itga5 f/f or Itga5 f/f ; Itga5 f/f ; P 0 - Cre tg/ + postnatal day (P)17 and P6 mice using RNeasy Plus Micro Kit (Qiagen) and TRIzol (Thermo Fisher), respectively. cDNA was synthesized using Super-Script III Reverse Transcriptase kit (Life Technologies).

    Article Title: In Vivo Generation of Neural Stem Cells Through Teratoma Formation.
    Article Snippet: 2 Pluripotent stem cells have the potential to differentiate into all cell types of the body in vitro through embryoid body formation or in vivo through teratoma formation.. Here, we attempted to generate in vivo neural stem cells (NSCs) differentiated through teratoma formation using Olig2-GFP transgenic embryonic stem cells (ESCs).. After 4 to 6 weeks of injection with Olig2-GFP transgenic ESCs, Olig2-GFP + NSCs were identified in teratomas formed in immunodeficient mice.

    Reverse Transcription:

    Article Title: Behavior of Mn-sod, PAL1, aos1 and HPL genes in soybean plants overexpressing the NmDef02 defensin
    Article Snippet: The cDNA was synthesized from 1μg of total RNA using an oligo-(dT) primer and the Super-Script III reverse transcriptase kit (Invitrogen, United States) according to the manufacturer’s instructions.

    Article Title: ABI3, a component of the WAVE2 complex, is potentially regulated by PI3K/AKT pathway
    Article Snippet: The full length cDNA of ABI3 (accession number NM_016428.2) was synthetized from a RNA isolated from a FTA sample using using Super-Script III Reverse Transcriptase kit with an oligo(dT)12–18 primer and 10 units of RNase inhibitor (Invitrogen, Life Technologies, Grand Island, NY), according to the manufacturers' recommendations.

    Article Title: α V integrins in Schwann cells promote attachment to axons, but are dispensable in vivo
    Article Snippet: RNA was isolated from purified SCs derived from desheathed sciatic nerves of Itga5 f/f ; Itga5 f/f or Itga5 f/f ; Itga5 f/f ; P 0 - Cre tg/ + postnatal day (P)17 and P6 mice using RNeasy Plus Micro Kit (Qiagen) and TRIzol (Thermo Fisher), respectively. cDNA was synthesized using Super-Script III Reverse Transcriptase kit (Life Technologies).

    Article Title: Aneuploidy as a cause of impaired chromatin silencing and mating-type specification in budding yeast
    Article Snippet: RNA was extracted as described above and cDNA was prepared from 2 μg of the resulting total RNA using the Super-Script III reverse transcriptase kit (Thermo Fisher Scientific, Waltham, MA).

    Article Title: Targeting the PRC2-dependent epigenetic program alleviates urinary tract infections
    Article Snippet: For quantitative real-time PCR (qRT-PCR) experiments, mRNA was reverse-transcribed into cDNA using the Super-Script III Reverse Transcriptase Kit (Invitrogen). qRT-PCR analyses were performed using SYBR Green (Roche) on an ABI-7500 detector (Applied BioSystems).

    Article Title: Alteration of circulating redox balance in coronavirus disease-19-induced acute respiratory distress syndrome
    Article Snippet: A260/A280 > 2 was evaluated to guarantee protein-free samples. cDNA was obtained using a random hexamer primer and a Super-Script III Reverse Transcriptase kit as described by the manufacturer (Invitrogen, Frederick, MD, USA).

    Article Title: α V integrins in Schwann cells promote attachment to axons, but are dispensable in vivo
    Article Snippet: Real-time quantitative PCR RNA was isolated from purified SCs derived from desheathed sciatic nerves of Itga5 f/f ; Itga5 f/f or Itga5 f/f ; Itga5 f/f ; P 0 - Cre tg/ + postnatal day (P)17 and P6 mice using RNeasy Plus Micro Kit (Qiagen) and TRIzol (Thermo Fisher), respectively. cDNA was synthesized using Super-Script III Reverse Transcriptase kit (Life Technologies).

    Article Title: In Vivo Generation of Neural Stem Cells Through Teratoma Formation.
    Article Snippet: 2 Pluripotent stem cells have the potential to differentiate into all cell types of the body in vitro through embryoid body formation or in vivo through teratoma formation.. Here, we attempted to generate in vivo neural stem cells (NSCs) differentiated through teratoma formation using Olig2-GFP transgenic embryonic stem cells (ESCs).. After 4 to 6 weeks of injection with Olig2-GFP transgenic ESCs, Olig2-GFP + NSCs were identified in teratomas formed in immunodeficient mice.

    Quantitative RT-PCR:

    Article Title: Behavior of Mn-sod, PAL1, aos1 and HPL genes in soybean plants overexpressing the NmDef02 defensin
    Article Snippet: The cDNA was synthesized from 1μg of total RNA using an oligo-(dT) primer and the Super-Script III reverse transcriptase kit (Invitrogen, United States) according to the manufacturer’s instructions.

    Article Title: ABI3, a component of the WAVE2 complex, is potentially regulated by PI3K/AKT pathway
    Article Snippet: The full length cDNA of ABI3 (accession number NM_016428.2) was synthetized from a RNA isolated from a FTA sample using using Super-Script III Reverse Transcriptase kit with an oligo(dT)12–18 primer and 10 units of RNase inhibitor (Invitrogen, Life Technologies, Grand Island, NY), according to the manufacturers' recommendations.

    Article Title: α V integrins in Schwann cells promote attachment to axons, but are dispensable in vivo
    Article Snippet: RNA was isolated from purified SCs derived from desheathed sciatic nerves of Itga5 f/f ; Itga5 f/f or Itga5 f/f ; Itga5 f/f ; P 0 - Cre tg/ + postnatal day (P)17 and P6 mice using RNeasy Plus Micro Kit (Qiagen) and TRIzol (Thermo Fisher), respectively. cDNA was synthesized using Super-Script III Reverse Transcriptase kit (Life Technologies).

    Article Title: Aneuploidy as a cause of impaired chromatin silencing and mating-type specification in budding yeast
    Article Snippet: RNA was extracted as described above and cDNA was prepared from 2 μg of the resulting total RNA using the Super-Script III reverse transcriptase kit (Thermo Fisher Scientific, Waltham, MA).

    Article Title: Targeting the PRC2-dependent epigenetic program alleviates urinary tract infections
    Article Snippet: For quantitative real-time PCR (qRT-PCR) experiments, mRNA was reverse-transcribed into cDNA using the Super-Script III Reverse Transcriptase Kit (Invitrogen). qRT-PCR analyses were performed using SYBR Green (Roche) on an ABI-7500 detector (Applied BioSystems).

    Article Title: Alteration of circulating redox balance in coronavirus disease-19-induced acute respiratory distress syndrome
    Article Snippet: A260/A280 > 2 was evaluated to guarantee protein-free samples. cDNA was obtained using a random hexamer primer and a Super-Script III Reverse Transcriptase kit as described by the manufacturer (Invitrogen, Frederick, MD, USA).

    Article Title: α V integrins in Schwann cells promote attachment to axons, but are dispensable in vivo
    Article Snippet: Real-time quantitative PCR RNA was isolated from purified SCs derived from desheathed sciatic nerves of Itga5 f/f ; Itga5 f/f or Itga5 f/f ; Itga5 f/f ; P 0 - Cre tg/ + postnatal day (P)17 and P6 mice using RNeasy Plus Micro Kit (Qiagen) and TRIzol (Thermo Fisher), respectively. cDNA was synthesized using Super-Script III Reverse Transcriptase kit (Life Technologies).

    Article Title: In Vivo Generation of Neural Stem Cells Through Teratoma Formation.
    Article Snippet: 2 Pluripotent stem cells have the potential to differentiate into all cell types of the body in vitro through embryoid body formation or in vivo through teratoma formation.. Here, we attempted to generate in vivo neural stem cells (NSCs) differentiated through teratoma formation using Olig2-GFP transgenic embryonic stem cells (ESCs).. After 4 to 6 weeks of injection with Olig2-GFP transgenic ESCs, Olig2-GFP + NSCs were identified in teratomas formed in immunodeficient mice.

    SYBR Green Assay:

    Article Title: Behavior of Mn-sod, PAL1, aos1 and HPL genes in soybean plants overexpressing the NmDef02 defensin
    Article Snippet: The cDNA was synthesized from 1μg of total RNA using an oligo-(dT) primer and the Super-Script III reverse transcriptase kit (Invitrogen, United States) according to the manufacturer’s instructions.

    Article Title: ABI3, a component of the WAVE2 complex, is potentially regulated by PI3K/AKT pathway
    Article Snippet: The full length cDNA of ABI3 (accession number NM_016428.2) was synthetized from a RNA isolated from a FTA sample using using Super-Script III Reverse Transcriptase kit with an oligo(dT)12–18 primer and 10 units of RNase inhibitor (Invitrogen, Life Technologies, Grand Island, NY), according to the manufacturers' recommendations.

    Article Title: α V integrins in Schwann cells promote attachment to axons, but are dispensable in vivo
    Article Snippet: RNA was isolated from purified SCs derived from desheathed sciatic nerves of Itga5 f/f ; Itga5 f/f or Itga5 f/f ; Itga5 f/f ; P 0 - Cre tg/ + postnatal day (P)17 and P6 mice using RNeasy Plus Micro Kit (Qiagen) and TRIzol (Thermo Fisher), respectively. cDNA was synthesized using Super-Script III Reverse Transcriptase kit (Life Technologies).

    Article Title: Aneuploidy as a cause of impaired chromatin silencing and mating-type specification in budding yeast
    Article Snippet: RNA was extracted as described above and cDNA was prepared from 2 μg of the resulting total RNA using the Super-Script III reverse transcriptase kit (Thermo Fisher Scientific, Waltham, MA).

    Article Title: Targeting the PRC2-dependent epigenetic program alleviates urinary tract infections
    Article Snippet: For quantitative real-time PCR (qRT-PCR) experiments, mRNA was reverse-transcribed into cDNA using the Super-Script III Reverse Transcriptase Kit (Invitrogen). qRT-PCR analyses were performed using SYBR Green (Roche) on an ABI-7500 detector (Applied BioSystems).

    Article Title: Alteration of circulating redox balance in coronavirus disease-19-induced acute respiratory distress syndrome
    Article Snippet: A260/A280 > 2 was evaluated to guarantee protein-free samples. cDNA was obtained using a random hexamer primer and a Super-Script III Reverse Transcriptase kit as described by the manufacturer (Invitrogen, Frederick, MD, USA).

    Article Title: α V integrins in Schwann cells promote attachment to axons, but are dispensable in vivo
    Article Snippet: Real-time quantitative PCR RNA was isolated from purified SCs derived from desheathed sciatic nerves of Itga5 f/f ; Itga5 f/f or Itga5 f/f ; Itga5 f/f ; P 0 - Cre tg/ + postnatal day (P)17 and P6 mice using RNeasy Plus Micro Kit (Qiagen) and TRIzol (Thermo Fisher), respectively. cDNA was synthesized using Super-Script III Reverse Transcriptase kit (Life Technologies).

    Article Title: In Vivo Generation of Neural Stem Cells Through Teratoma Formation.
    Article Snippet: 2 Pluripotent stem cells have the potential to differentiate into all cell types of the body in vitro through embryoid body formation or in vivo through teratoma formation.. Here, we attempted to generate in vivo neural stem cells (NSCs) differentiated through teratoma formation using Olig2-GFP transgenic embryonic stem cells (ESCs).. After 4 to 6 weeks of injection with Olig2-GFP transgenic ESCs, Olig2-GFP + NSCs were identified in teratomas formed in immunodeficient mice.

    Isolation:

    Article Title: Behavior of Mn-sod, PAL1, aos1 and HPL genes in soybean plants overexpressing the NmDef02 defensin
    Article Snippet: The cDNA was synthesized from 1μg of total RNA using an oligo-(dT) primer and the Super-Script III reverse transcriptase kit (Invitrogen, United States) according to the manufacturer’s instructions.

    Article Title: ABI3, a component of the WAVE2 complex, is potentially regulated by PI3K/AKT pathway
    Article Snippet: The full length cDNA of ABI3 (accession number NM_016428.2) was synthetized from a RNA isolated from a FTA sample using using Super-Script III Reverse Transcriptase kit with an oligo(dT)12–18 primer and 10 units of RNase inhibitor (Invitrogen, Life Technologies, Grand Island, NY), according to the manufacturers' recommendations.

    Article Title: α V integrins in Schwann cells promote attachment to axons, but are dispensable in vivo
    Article Snippet: RNA was isolated from purified SCs derived from desheathed sciatic nerves of Itga5 f/f ; Itga5 f/f or Itga5 f/f ; Itga5 f/f ; P 0 - Cre tg/ + postnatal day (P)17 and P6 mice using RNeasy Plus Micro Kit (Qiagen) and TRIzol (Thermo Fisher), respectively. cDNA was synthesized using Super-Script III Reverse Transcriptase kit (Life Technologies).

    Article Title: Aneuploidy as a cause of impaired chromatin silencing and mating-type specification in budding yeast
    Article Snippet: RNA was extracted as described above and cDNA was prepared from 2 μg of the resulting total RNA using the Super-Script III reverse transcriptase kit (Thermo Fisher Scientific, Waltham, MA).

    Article Title: Targeting the PRC2-dependent epigenetic program alleviates urinary tract infections
    Article Snippet: For quantitative real-time PCR (qRT-PCR) experiments, mRNA was reverse-transcribed into cDNA using the Super-Script III Reverse Transcriptase Kit (Invitrogen). qRT-PCR analyses were performed using SYBR Green (Roche) on an ABI-7500 detector (Applied BioSystems).

    Article Title: Alteration of circulating redox balance in coronavirus disease-19-induced acute respiratory distress syndrome
    Article Snippet: A260/A280 > 2 was evaluated to guarantee protein-free samples. cDNA was obtained using a random hexamer primer and a Super-Script III Reverse Transcriptase kit as described by the manufacturer (Invitrogen, Frederick, MD, USA).

    Article Title: α V integrins in Schwann cells promote attachment to axons, but are dispensable in vivo
    Article Snippet: Real-time quantitative PCR RNA was isolated from purified SCs derived from desheathed sciatic nerves of Itga5 f/f ; Itga5 f/f or Itga5 f/f ; Itga5 f/f ; P 0 - Cre tg/ + postnatal day (P)17 and P6 mice using RNeasy Plus Micro Kit (Qiagen) and TRIzol (Thermo Fisher), respectively. cDNA was synthesized using Super-Script III Reverse Transcriptase kit (Life Technologies).

    Article Title: In Vivo Generation of Neural Stem Cells Through Teratoma Formation.
    Article Snippet: 2 Pluripotent stem cells have the potential to differentiate into all cell types of the body in vitro through embryoid body formation or in vivo through teratoma formation.. Here, we attempted to generate in vivo neural stem cells (NSCs) differentiated through teratoma formation using Olig2-GFP transgenic embryonic stem cells (ESCs).. After 4 to 6 weeks of injection with Olig2-GFP transgenic ESCs, Olig2-GFP + NSCs were identified in teratomas formed in immunodeficient mice.

    Purification:

    Article Title: Behavior of Mn-sod, PAL1, aos1 and HPL genes in soybean plants overexpressing the NmDef02 defensin
    Article Snippet: The cDNA was synthesized from 1μg of total RNA using an oligo-(dT) primer and the Super-Script III reverse transcriptase kit (Invitrogen, United States) according to the manufacturer’s instructions.

    Article Title: ABI3, a component of the WAVE2 complex, is potentially regulated by PI3K/AKT pathway
    Article Snippet: The full length cDNA of ABI3 (accession number NM_016428.2) was synthetized from a RNA isolated from a FTA sample using using Super-Script III Reverse Transcriptase kit with an oligo(dT)12–18 primer and 10 units of RNase inhibitor (Invitrogen, Life Technologies, Grand Island, NY), according to the manufacturers' recommendations.

    Article Title: α V integrins in Schwann cells promote attachment to axons, but are dispensable in vivo
    Article Snippet: RNA was isolated from purified SCs derived from desheathed sciatic nerves of Itga5 f/f ; Itga5 f/f or Itga5 f/f ; Itga5 f/f ; P 0 - Cre tg/ + postnatal day (P)17 and P6 mice using RNeasy Plus Micro Kit (Qiagen) and TRIzol (Thermo Fisher), respectively. cDNA was synthesized using Super-Script III Reverse Transcriptase kit (Life Technologies).

    Article Title: Aneuploidy as a cause of impaired chromatin silencing and mating-type specification in budding yeast
    Article Snippet: RNA was extracted as described above and cDNA was prepared from 2 μg of the resulting total RNA using the Super-Script III reverse transcriptase kit (Thermo Fisher Scientific, Waltham, MA).

    Article Title: Targeting the PRC2-dependent epigenetic program alleviates urinary tract infections
    Article Snippet: For quantitative real-time PCR (qRT-PCR) experiments, mRNA was reverse-transcribed into cDNA using the Super-Script III Reverse Transcriptase Kit (Invitrogen). qRT-PCR analyses were performed using SYBR Green (Roche) on an ABI-7500 detector (Applied BioSystems).

    Article Title: Alteration of circulating redox balance in coronavirus disease-19-induced acute respiratory distress syndrome
    Article Snippet: A260/A280 > 2 was evaluated to guarantee protein-free samples. cDNA was obtained using a random hexamer primer and a Super-Script III Reverse Transcriptase kit as described by the manufacturer (Invitrogen, Frederick, MD, USA).

    Article Title: α V integrins in Schwann cells promote attachment to axons, but are dispensable in vivo
    Article Snippet: Real-time quantitative PCR RNA was isolated from purified SCs derived from desheathed sciatic nerves of Itga5 f/f ; Itga5 f/f or Itga5 f/f ; Itga5 f/f ; P 0 - Cre tg/ + postnatal day (P)17 and P6 mice using RNeasy Plus Micro Kit (Qiagen) and TRIzol (Thermo Fisher), respectively. cDNA was synthesized using Super-Script III Reverse Transcriptase kit (Life Technologies).

    Article Title: In Vivo Generation of Neural Stem Cells Through Teratoma Formation.
    Article Snippet: 2 Pluripotent stem cells have the potential to differentiate into all cell types of the body in vitro through embryoid body formation or in vivo through teratoma formation.. Here, we attempted to generate in vivo neural stem cells (NSCs) differentiated through teratoma formation using Olig2-GFP transgenic embryonic stem cells (ESCs).. After 4 to 6 weeks of injection with Olig2-GFP transgenic ESCs, Olig2-GFP + NSCs were identified in teratomas formed in immunodeficient mice.

    Derivative Assay:

    Article Title: Behavior of Mn-sod, PAL1, aos1 and HPL genes in soybean plants overexpressing the NmDef02 defensin
    Article Snippet: The cDNA was synthesized from 1μg of total RNA using an oligo-(dT) primer and the Super-Script III reverse transcriptase kit (Invitrogen, United States) according to the manufacturer’s instructions.

    Article Title: ABI3, a component of the WAVE2 complex, is potentially regulated by PI3K/AKT pathway
    Article Snippet: The full length cDNA of ABI3 (accession number NM_016428.2) was synthetized from a RNA isolated from a FTA sample using using Super-Script III Reverse Transcriptase kit with an oligo(dT)12–18 primer and 10 units of RNase inhibitor (Invitrogen, Life Technologies, Grand Island, NY), according to the manufacturers' recommendations.

    Article Title: α V integrins in Schwann cells promote attachment to axons, but are dispensable in vivo
    Article Snippet: RNA was isolated from purified SCs derived from desheathed sciatic nerves of Itga5 f/f ; Itga5 f/f or Itga5 f/f ; Itga5 f/f ; P 0 - Cre tg/ + postnatal day (P)17 and P6 mice using RNeasy Plus Micro Kit (Qiagen) and TRIzol (Thermo Fisher), respectively. cDNA was synthesized using Super-Script III Reverse Transcriptase kit (Life Technologies).

    Article Title: Aneuploidy as a cause of impaired chromatin silencing and mating-type specification in budding yeast
    Article Snippet: RNA was extracted as described above and cDNA was prepared from 2 μg of the resulting total RNA using the Super-Script III reverse transcriptase kit (Thermo Fisher Scientific, Waltham, MA).

    Article Title: Targeting the PRC2-dependent epigenetic program alleviates urinary tract infections
    Article Snippet: For quantitative real-time PCR (qRT-PCR) experiments, mRNA was reverse-transcribed into cDNA using the Super-Script III Reverse Transcriptase Kit (Invitrogen). qRT-PCR analyses were performed using SYBR Green (Roche) on an ABI-7500 detector (Applied BioSystems).

    Article Title: Alteration of circulating redox balance in coronavirus disease-19-induced acute respiratory distress syndrome
    Article Snippet: A260/A280 > 2 was evaluated to guarantee protein-free samples. cDNA was obtained using a random hexamer primer and a Super-Script III Reverse Transcriptase kit as described by the manufacturer (Invitrogen, Frederick, MD, USA).

    Article Title: α V integrins in Schwann cells promote attachment to axons, but are dispensable in vivo
    Article Snippet: Real-time quantitative PCR RNA was isolated from purified SCs derived from desheathed sciatic nerves of Itga5 f/f ; Itga5 f/f or Itga5 f/f ; Itga5 f/f ; P 0 - Cre tg/ + postnatal day (P)17 and P6 mice using RNeasy Plus Micro Kit (Qiagen) and TRIzol (Thermo Fisher), respectively. cDNA was synthesized using Super-Script III Reverse Transcriptase kit (Life Technologies).

    Article Title: In Vivo Generation of Neural Stem Cells Through Teratoma Formation.
    Article Snippet: 2 Pluripotent stem cells have the potential to differentiate into all cell types of the body in vitro through embryoid body formation or in vivo through teratoma formation.. Here, we attempted to generate in vivo neural stem cells (NSCs) differentiated through teratoma formation using Olig2-GFP transgenic embryonic stem cells (ESCs).. After 4 to 6 weeks of injection with Olig2-GFP transgenic ESCs, Olig2-GFP + NSCs were identified in teratomas formed in immunodeficient mice.

    Synthesized:

    Article Title: Behavior of Mn-sod, PAL1, aos1 and HPL genes in soybean plants overexpressing the NmDef02 defensin
    Article Snippet: The cDNA was synthesized from 1μg of total RNA using an oligo-(dT) primer and the Super-Script III reverse transcriptase kit (Invitrogen, United States) according to the manufacturer’s instructions.

    Article Title: ABI3, a component of the WAVE2 complex, is potentially regulated by PI3K/AKT pathway
    Article Snippet: The full length cDNA of ABI3 (accession number NM_016428.2) was synthetized from a RNA isolated from a FTA sample using using Super-Script III Reverse Transcriptase kit with an oligo(dT)12–18 primer and 10 units of RNase inhibitor (Invitrogen, Life Technologies, Grand Island, NY), according to the manufacturers' recommendations.

    Article Title: α V integrins in Schwann cells promote attachment to axons, but are dispensable in vivo
    Article Snippet: RNA was isolated from purified SCs derived from desheathed sciatic nerves of Itga5 f/f ; Itga5 f/f or Itga5 f/f ; Itga5 f/f ; P 0 - Cre tg/ + postnatal day (P)17 and P6 mice using RNeasy Plus Micro Kit (Qiagen) and TRIzol (Thermo Fisher), respectively. cDNA was synthesized using Super-Script III Reverse Transcriptase kit (Life Technologies).

    Article Title: Aneuploidy as a cause of impaired chromatin silencing and mating-type specification in budding yeast
    Article Snippet: RNA was extracted as described above and cDNA was prepared from 2 μg of the resulting total RNA using the Super-Script III reverse transcriptase kit (Thermo Fisher Scientific, Waltham, MA).

    Article Title: Targeting the PRC2-dependent epigenetic program alleviates urinary tract infections
    Article Snippet: For quantitative real-time PCR (qRT-PCR) experiments, mRNA was reverse-transcribed into cDNA using the Super-Script III Reverse Transcriptase Kit (Invitrogen). qRT-PCR analyses were performed using SYBR Green (Roche) on an ABI-7500 detector (Applied BioSystems).

    Article Title: Alteration of circulating redox balance in coronavirus disease-19-induced acute respiratory distress syndrome
    Article Snippet: A260/A280 > 2 was evaluated to guarantee protein-free samples. cDNA was obtained using a random hexamer primer and a Super-Script III Reverse Transcriptase kit as described by the manufacturer (Invitrogen, Frederick, MD, USA).

    Article Title: α V integrins in Schwann cells promote attachment to axons, but are dispensable in vivo
    Article Snippet: Real-time quantitative PCR RNA was isolated from purified SCs derived from desheathed sciatic nerves of Itga5 f/f ; Itga5 f/f or Itga5 f/f ; Itga5 f/f ; P 0 - Cre tg/ + postnatal day (P)17 and P6 mice using RNeasy Plus Micro Kit (Qiagen) and TRIzol (Thermo Fisher), respectively. cDNA was synthesized using Super-Script III Reverse Transcriptase kit (Life Technologies).

    Article Title: In Vivo Generation of Neural Stem Cells Through Teratoma Formation.
    Article Snippet: 2 Pluripotent stem cells have the potential to differentiate into all cell types of the body in vitro through embryoid body formation or in vivo through teratoma formation.. Here, we attempted to generate in vivo neural stem cells (NSCs) differentiated through teratoma formation using Olig2-GFP transgenic embryonic stem cells (ESCs).. After 4 to 6 weeks of injection with Olig2-GFP transgenic ESCs, Olig2-GFP + NSCs were identified in teratomas formed in immunodeficient mice.



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